EXPERIMENTAL EVALUATION OF MG-63 CELLS BIOLOGICAL RESPONSE TO LOW INTENSITY ULTRASOUNDS EXPOSURE

Abstract

The purpose of this study is to evaluate effect of low intensity ultrasounds (US) on MG-63 osteosarcoma cell lines. It is known that low intensity ultrasound can induce mild thermal, apoptosis and sono-mechanical effects. However, response to ultrasonic treatment depends at a great extent on the type of tested biological tissues. In order to find the frequency or the range of frequency yielding most significant biological effects on MG-63 cells, experimental tests are performed by sonicating MG-63 cells at fixed frequency. Cell cultures are grown in 60 mm Petri dishes and then exposed to US for 3 minutes. US waves are generated by a SonoPore KTAC-4000 sonoporator and then transferred to specimens by KP-S20 probes. The selected frequencies are 400, 510, 582, 800 and 1.000 kHz. Cell viability is measured after sonication using a TC20 cell counter. The number of counted cells is found to decrease with sonication frequency. In particular, it drops to about 30% at 1.000 KHz. Interpretations of experimental evidence are attempted in the article.